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anti gadph  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti gadph
    Anti Gadph, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gadph/pmc13053778-8-0-2
    Average 86 stars, based on 1 article reviews
    anti gadph - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: SARS-CoV-2 Nonstructural Protein 1 Suppresses Host Transcription by Reducing RNA Polymerase II Levels
    Article Snippet: Images were acquired using ZEISS Axioscope 5 and ZEISS LSM 900 confocal microscope and analyzed with ImageJ software. .. We used following antibodies for Western blot, immunoprecipitation and immunofluorescence staining: β-actin (4970, CST), GADPH (2118, CST), GFP (ab290, Abcam), mCherry (ab183628, Abcam), NSP1 (ab284631, Abcam), Lamin A/C (4777, CST), Pol II (05-623, Sigma Aldrich), Flag (14793, CST), and Rabbit IgG (66362, CST). .. Following transfection, H1299 cells were harvested, washed once with ice-cold PBS, and lysed on ice for 30 min at a density of 1 × 10 6 cells per 200 μL lysis buffer supplemented with protease and phosphatase inhibitors (P2179S, Beyotime).

    Article Title: SARS-CoV-2 nonstructural protein 1 suppresses host transcription by reducing RNA polymerase II levels
    Article Snippet: Images were acquired using ZEISS Axioscope 5 and ZEISS LSM 900 confocal microscope and analyzed with ImageJ software. .. We used following antibodies for Western blot, immunoprecipitation and immunofluorescence staining: β-actin (4970, CST), GADPH (2118, CST), GFP (ab290, Abcam), mCherry (ab183628, Abcam), NSP1 (ab284631, Abcam), Lamin A/C (4777, CST), Pol II (05-623, Sigma Aldrich), Flag (14793, CST), and Rabbit IgG (66362, CST). .. Following transfection, H1299 cells were harvested, washed once with ice-cold PBS, and lysed on ice for 30 min at a density of 1 × 10 6 cells per 200 μL lysis buffer supplemented with protease and phosphatase inhibitors (P2179S, Beyotime).

    Immunoprecipitation:

    Article Title: SARS-CoV-2 Nonstructural Protein 1 Suppresses Host Transcription by Reducing RNA Polymerase II Levels
    Article Snippet: Images were acquired using ZEISS Axioscope 5 and ZEISS LSM 900 confocal microscope and analyzed with ImageJ software. .. We used following antibodies for Western blot, immunoprecipitation and immunofluorescence staining: β-actin (4970, CST), GADPH (2118, CST), GFP (ab290, Abcam), mCherry (ab183628, Abcam), NSP1 (ab284631, Abcam), Lamin A/C (4777, CST), Pol II (05-623, Sigma Aldrich), Flag (14793, CST), and Rabbit IgG (66362, CST). .. Following transfection, H1299 cells were harvested, washed once with ice-cold PBS, and lysed on ice for 30 min at a density of 1 × 10 6 cells per 200 μL lysis buffer supplemented with protease and phosphatase inhibitors (P2179S, Beyotime).

    Article Title: SARS-CoV-2 nonstructural protein 1 suppresses host transcription by reducing RNA polymerase II levels
    Article Snippet: Images were acquired using ZEISS Axioscope 5 and ZEISS LSM 900 confocal microscope and analyzed with ImageJ software. .. We used following antibodies for Western blot, immunoprecipitation and immunofluorescence staining: β-actin (4970, CST), GADPH (2118, CST), GFP (ab290, Abcam), mCherry (ab183628, Abcam), NSP1 (ab284631, Abcam), Lamin A/C (4777, CST), Pol II (05-623, Sigma Aldrich), Flag (14793, CST), and Rabbit IgG (66362, CST). .. Following transfection, H1299 cells were harvested, washed once with ice-cold PBS, and lysed on ice for 30 min at a density of 1 × 10 6 cells per 200 μL lysis buffer supplemented with protease and phosphatase inhibitors (P2179S, Beyotime).

    Immunofluorescence:

    Article Title: SARS-CoV-2 Nonstructural Protein 1 Suppresses Host Transcription by Reducing RNA Polymerase II Levels
    Article Snippet: Images were acquired using ZEISS Axioscope 5 and ZEISS LSM 900 confocal microscope and analyzed with ImageJ software. .. We used following antibodies for Western blot, immunoprecipitation and immunofluorescence staining: β-actin (4970, CST), GADPH (2118, CST), GFP (ab290, Abcam), mCherry (ab183628, Abcam), NSP1 (ab284631, Abcam), Lamin A/C (4777, CST), Pol II (05-623, Sigma Aldrich), Flag (14793, CST), and Rabbit IgG (66362, CST). .. Following transfection, H1299 cells were harvested, washed once with ice-cold PBS, and lysed on ice for 30 min at a density of 1 × 10 6 cells per 200 μL lysis buffer supplemented with protease and phosphatase inhibitors (P2179S, Beyotime).

    Article Title: SARS-CoV-2 nonstructural protein 1 suppresses host transcription by reducing RNA polymerase II levels
    Article Snippet: Images were acquired using ZEISS Axioscope 5 and ZEISS LSM 900 confocal microscope and analyzed with ImageJ software. .. We used following antibodies for Western blot, immunoprecipitation and immunofluorescence staining: β-actin (4970, CST), GADPH (2118, CST), GFP (ab290, Abcam), mCherry (ab183628, Abcam), NSP1 (ab284631, Abcam), Lamin A/C (4777, CST), Pol II (05-623, Sigma Aldrich), Flag (14793, CST), and Rabbit IgG (66362, CST). .. Following transfection, H1299 cells were harvested, washed once with ice-cold PBS, and lysed on ice for 30 min at a density of 1 × 10 6 cells per 200 μL lysis buffer supplemented with protease and phosphatase inhibitors (P2179S, Beyotime).

    Staining:

    Article Title: SARS-CoV-2 Nonstructural Protein 1 Suppresses Host Transcription by Reducing RNA Polymerase II Levels
    Article Snippet: Images were acquired using ZEISS Axioscope 5 and ZEISS LSM 900 confocal microscope and analyzed with ImageJ software. .. We used following antibodies for Western blot, immunoprecipitation and immunofluorescence staining: β-actin (4970, CST), GADPH (2118, CST), GFP (ab290, Abcam), mCherry (ab183628, Abcam), NSP1 (ab284631, Abcam), Lamin A/C (4777, CST), Pol II (05-623, Sigma Aldrich), Flag (14793, CST), and Rabbit IgG (66362, CST). .. Following transfection, H1299 cells were harvested, washed once with ice-cold PBS, and lysed on ice for 30 min at a density of 1 × 10 6 cells per 200 μL lysis buffer supplemented with protease and phosphatase inhibitors (P2179S, Beyotime).

    Article Title: SARS-CoV-2 nonstructural protein 1 suppresses host transcription by reducing RNA polymerase II levels
    Article Snippet: Images were acquired using ZEISS Axioscope 5 and ZEISS LSM 900 confocal microscope and analyzed with ImageJ software. .. We used following antibodies for Western blot, immunoprecipitation and immunofluorescence staining: β-actin (4970, CST), GADPH (2118, CST), GFP (ab290, Abcam), mCherry (ab183628, Abcam), NSP1 (ab284631, Abcam), Lamin A/C (4777, CST), Pol II (05-623, Sigma Aldrich), Flag (14793, CST), and Rabbit IgG (66362, CST). .. Following transfection, H1299 cells were harvested, washed once with ice-cold PBS, and lysed on ice for 30 min at a density of 1 × 10 6 cells per 200 μL lysis buffer supplemented with protease and phosphatase inhibitors (P2179S, Beyotime).



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